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. 2019 Apr 20;101(1):188–199. doi: 10.1093/biolre/ioz071

Figure 7.

Figure 7.

IFT74 regulates expression levels of some IFT and flagellar proteins. (A) Examination of selective IFT protein expression levels in the control and conditional Ift74 KO mice by western blot. Compared to the controls, the expression levels of IFT27, IFT57, IFT81, IFT88, and IFT140, but not IFT20 and IFT25 are reduced in the conditional Ift74 KO mice. (a) Representative western blot result; (b) quantitative analysis of selective IFT protein expression. (B) Examination of testicular expression levels of ODF2, a component of sperm tail outer dense fibers, AKAP4, a major component of fibrous sheath, and SPAG16L, a component of axonemal central apparatus protein in the control and conditional Ift74 KO mice. There was no difference in ODF2 and SPAG16L expression. However, the processed AKAP4 was significantly reduced in the conditional Ift74 KO mice. β-ACTIN was used as controls. (a) Representative western blot result; (b) quantitative analysis of ODF2 and SPAG16L; (c) quantitative analysis of AKAP4. Data are expressed as means ± SD (n = 3). *P < 0.05 compared with the control mice.