Table 1.
Antibodies information.
| Name | Catalogue number | Purpose | Dilution |
|---|---|---|---|
| Anti-IFT25 | 15732-1-AP, ProteinTech | Western Blot | 1:2000 |
| Anti-IFT74 | AS27620e, Antibody Verify | Western Blot, IF | 1:2000 for Western Blot; 1:200 for IF |
| Anti-IFT81 | 11744-1-AP, ProteinTech | Western Blot | 1:1000 |
| β-actin | 4967 S, Cell Signaling | Western Blot | 1:2000 |
| Anti-IFT20 | Dr. Pazour's laboratory | Western Blot | 1:2000 |
| Anti-IFT27 | Dr. Pazour's laboratory | Western Blot | 1:2000 |
| Anti-IFT57 | Dr. Pazour's laboratory | Western Blot | 1:2000 |
| Anti-IFT88 | Dr. Pazour's laboratory | Western Blot | 1:2000 |
| Anti-IFT140 | Dr. Pazour's laboratory | Western Blot | 1:2000 |
| Anti-AKAP4 | Dr. George Gerton at University of Pennsylvania | Western Blot | 1:4000 |
| Anti-ODF2 | 12058-1-AP, ProteinTech | Western Blot | 1:800 |
| Anti-SPAG16L | Dr. Zhibing Zhang's laboratory | Western Blot | 1:1000 |
| Anti-α-tubulin | T9026-2ML, Sigma | IF | 1:200 |
| Lectin PNA | L21409, InvitrogenTM | IF | 20 μg/ml |