Figure 4.

Knockdown of NOX4 inhibits ROS generation, epithelial mesenchymal transition and MMP7 expression of gastric cancer cells. MKN-45 and AGS cells were transfected with siRNA-Negative Control or siRNA-NOX4 for 24 h. A. ROS generation by normal gastric epithelial cell line, GES-1, gastric cancer cell line, MKN-45 and AGS cells as measured by flow cytometry using H₂DCFDA. B. ROS generation by gastric cancer cells and gastric cancer cells with NOX4 knockdown as measured by flow cytometry using H₂DCFDA. C. The expression of NOX4, MMP-2, MMP-7, MMP-9, MMP-13, Nanog, Bmi-1, Notch3, Vimentin, ZO-1, N-Cadherin and E-Cadherin in GC cells and GC cells with NOX4 knockdown was measured by immunoblotting with the indicated antibodies. GAPDH served as a loading control.