Figure 5.

CRIP1 silencing alleviated the effects of zinc supplementation in medium. (A, B) The migration and invasion capabilities of SW620 and LoVo cells treated with 50-µM Zn²⁺ after transfected with siNC or siCRIP1 for 24 h detected using Transwell assays. The upper graphs provide the representative graph results, whereas the lower graphs provide the statistical results. (C, D) The expression levels of EMT markers (C) and the phosphorylation levels of p-GSK3B and p-mTOR (D) in SW620 and LoVo cells treated with 50 µM Zn²⁺ after being transfected with siNC or siCRIP1 for 24 h. On the left are the representative graphs of the western blot. On the right are the statistical results of the relative protein expression levels. *P < 0.05, when compared to the siNC+Zn²⁺ group.