Figure 1.
Diosgenin inhibits cell growth and induces apoptosis and cell cycle arrest. A: PC-3 cells were seeded in 96-well plates and cultured overnight, followed by treatment with 0, 25, 50, 75, or 100 µM of diosgenin. After treatment with diosgenin for 48 h and 72 h, the MTT assay was carried out to measure cell growth. *P < 0.05, when compared to the control group. B: PC-3 cells (1×105 cells/well) were seeded in 6-well platesand cultured overnight, following by treatment with 50 µM and 75 µM diosgenin for 48 h. Annexin V-FITC/PI staining and flow cytometry were used to measure apoptotic cell death. C: Diosgenin-induced PC-3 cell cycle arrest was measured using PI staining and flow cytometry.