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NEDD4 siRNA enhances diosgenin-inhibited NEDD4 level. (A) The expression of NEDD4, p73, LATS1, and pAkt was determined by Western blotting analysis in PC-3 cells after diosgenin exposure in combination with NEDD4 siRNA transfection. (B) Quantification of the data presented in (A). *P < 0.05, when compared with control; #P < 0.05, when compared to diosgenin treatment alone or NEDD4 siRNA transfection alone.
