Figure 5.
The resistance of P. aeruginosa to amikacin in diabetic mice treated with insulin was higher than that in nondiabetic mice and untreated diabetic mice. In particular, in the PAO1/Plac-yhjH-infected group, the resistance of diabetic mice treated with insulin was significantly higher than that of nondiabetic mice or untreated diabetic mice at days 4 and 16. The mice were anesthetized, and the gauze pads were removed from the wounds and treated with amikacin or PBS on the day of surgery and at 4-day intervals post surgery. The amikacin resistance of P. aeruginosa in the wounds was calculated by the following equation: (Ra CFU/g)/(Rp CFU/g) × 100, where Ra CFU/g refers to gauze pads treated with amikacin, and Rp CFU/g refers to gauze pads treated with PBS. One-way analysis of variance (ANOVA) followed by the Tukey-Kramer multiple comparison test was used. Note: *P < 0.05 compared with the nondiabetic group, **P < 0.01 compared with the nondiabetic group, ΔP < 0.05 compared with the diabetic group, ΔΔP < 0.01 compared with the diabetic group.