Figure 2.

The effect of embryo culture on Slc2a expression in the mouse blastocyst. The mean ± S.E.M of normalized expression of SLC2A in the blastocysts derived from zygote stage in vitro culture in KSOM with or without amino acids, compared to the blastocyst collected from reproductive tracts (fresh blastocyst), was analyzed by TaqMan gene expression assay. The data were representative of five independent replicates, each with 10 blastocysts per treatment. *P < 0.001, compared to corresponding fresh blastocyst; **P < 0.01, compared to the corresponding treatments without amino acids.