Figure 6.

Effect of insulin on SLC2A4-dependent development and glucose consumption. A. The outcomes of blastocyst formation and cell accumulation in blastocysts derived after zygote culture for 96 h in KSOM medium with treatments including (1) with or without 10 µg/mL insulin and (2) with or without indinavir sulfate. The results are representative of three independent replicates with at least 70 embryos for each treatment. P > 0.05 for the effect of insulin on blastocyst formation rate and cell number; P < 0.001 for indinavir sulfate; P < 0.05 for interaction between indinavir sulfate and insulin. B. The proportion of glucose remaining in the culture media collected at four developmental time points. The results are representative of six independent replicates. In each replicate, 30 µL of media from five groups of same treatment were collected and stored at -20°C. The glucose assay was performed after 96 h of culture.