Figure 3.

miR-361-5p targeted 3’-UTR of LncRNA MEG3. A. The putative miR-361-5p binding sequence of the wild type and mutation sequence of MEG3. B. Luciferase activity of a luciferase reporter plasmid (pluc) containing wild-type or mutant MEG3 3’UTR co-transfected with miR-361-5p was determined by the dual luciferase assay. C. The expression of miR-361-5p was detected in different groups by using RT-PCR. D. Cellular lysates from VSMC cells were used for RIP with an Ago2 antibody and IgG antibody. The levels of MEG3 and miR-361-5p were detected by qRT-PCR. Data were represented as mean ± SEM. *P<0.05, **P<0.01, and ***P<0.001.