Table 1.
Genes | Accession | Forward | Reverse | Size | Tm | Cycles |
---|---|---|---|---|---|---|
GAPDH | NM_002046 | cgagatccctccaaaatcaagt | tgaggctgttgtcatacttctcat | 196 | 56 | 30 |
Sox17 | NM_022454 | acgctttcatggtgtgggctaag | gtcagcgccttccacgacttg | 112 | 60 | 40 |
Foxa2 | NM_021784 | attgctggtcgtttgttgtg | tacgtgttcatgccgttcat | 187 | 60 | 40 |
Nkx6.1 | NM_006168 | tcttctggcccggagtgatg | ggaaaaagtgggtctcgtgtgtt | 125 | 66 | 30 |
Ngn3 | NM_020999 | ttcgcccacaactacatc | gacagacaggtcctttcac | 282 | 56 | 30 |
Nkx2.2 | NM_002509 | cttctacgacagcagcgaca | tgtcattgtccggtgactcg | 155 | 56 | 30 |
Gcg | NM_002054 | aagttcccaaagagggcttg | agctgccttgtaccagcatt | 126 | 56 | 30 |
Sst | NM_001048 | acgcaaagctggctgcaaga | gggggcgagggatcagaggt | 100 | 56 | 30 |
Ppy | NM_002722 | aatgccacaccagagcagat | cgtaggagacagaaggtggc | 192 | 56 | 30 |
MAFA | NM_201589 | cttcagcaaggaggaggtcatc | tctcgctctccagaatgtgcc | 119 | 60 | 30 |
PC1 | NM_000439 | gtgacggatgctattgaggc | tgatggagatggtgtagatgct | 262 | 56 | 30 |
PC2 | NM_002594 | tgccgaagcaagttacgact | aacttctcctgcacatcggg | 103 | 56 | 30 |
Kir6.2 | NM_000525 | ggacccaggtggaggtaagg | ctctcggtgggcaccttctc | 103 | 56 | 30 |
Sur1 | NM_001287174 | gaggctacttcacgtggacc | ctatctcgctgtcaggaaggc | 198 | 56 | 30 |
Glut2 | NM_000340 | gtcactgggaccctggtttt | gtcatccagtggaacacccaa | 141 | 56 | 30 |
Pdx1 | NM_000209 | atgaagtctaccaaagctcacgc | tctctcggtcaagttcaacatga | 201 | 60 | 40 |
Ins | NM_000207 | acgaggcttcttctacacaccca | tgttccacaatgccacgcttc | 147 | 60 | 40 |