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. 2019 Jun 15;11(6):3490–3504.

Table 1.

Primers for PCR and qPCR

Genes Accession Forward Reverse Size Tm Cycles
GAPDH NM_002046 cgagatccctccaaaatcaagt tgaggctgttgtcatacttctcat 196 56 30
Sox17 NM_022454 acgctttcatggtgtgggctaag gtcagcgccttccacgacttg 112 60 40
Foxa2 NM_021784 attgctggtcgtttgttgtg tacgtgttcatgccgttcat 187 60 40
Nkx6.1 NM_006168 tcttctggcccggagtgatg ggaaaaagtgggtctcgtgtgtt 125 66 30
Ngn3 NM_020999 ttcgcccacaactacatc gacagacaggtcctttcac 282 56 30
Nkx2.2 NM_002509 cttctacgacagcagcgaca tgtcattgtccggtgactcg 155 56 30
Gcg NM_002054 aagttcccaaagagggcttg agctgccttgtaccagcatt 126 56 30
Sst NM_001048 acgcaaagctggctgcaaga gggggcgagggatcagaggt 100 56 30
Ppy NM_002722 aatgccacaccagagcagat cgtaggagacagaaggtggc 192 56 30
MAFA NM_201589 cttcagcaaggaggaggtcatc tctcgctctccagaatgtgcc 119 60 30
PC1 NM_000439 gtgacggatgctattgaggc tgatggagatggtgtagatgct 262 56 30
PC2 NM_002594 tgccgaagcaagttacgact aacttctcctgcacatcggg 103 56 30
Kir6.2 NM_000525 ggacccaggtggaggtaagg ctctcggtgggcaccttctc 103 56 30
Sur1 NM_001287174 gaggctacttcacgtggacc ctatctcgctgtcaggaaggc 198 56 30
Glut2 NM_000340 gtcactgggaccctggtttt gtcatccagtggaacacccaa 141 56 30
Pdx1 NM_000209 atgaagtctaccaaagctcacgc tctctcggtcaagttcaacatga 201 60 40
Ins NM_000207 acgaggcttcttctacacaccca tgttccacaatgccacgcttc 147 60 40