Figure 4.

Immunofluorescence staining of SaOS-2 cells after incubation without TKI (control, upper row), IMA (1 µM, middle row) or NIL (1 µM, lower row) for 25 days, respectively. Cells were stained with RANKL (red), OPG (purple), and DAPI (blue). One representative image is shown per treatment. Each experiment was performed three times. IMA, imatinib; NIL, nilotinib; RANKL, receptor activator of nuclear factor κB ligand; OPG, osteoprotegerin.