Table I.
mTOR, RPTOR and RICTOR primer sequences.
| Primer | Sequence | Amplicon size |
|---|---|---|
| mTOR | 146 (bp) | |
| F | 5′-AGCATCGGATGCTTAGGAGTGG-3′ | |
| R | 5′-CAGCCAGTCATCTTTGGAGACC-3′ | |
| RPTOR | 148 (bp) | |
| F | 5′-GATCGTCAACAGCTATCACACGG-3′ | |
| R | 5′-CGAGTCGAAGTTCTGCCAGATC-3′ | |
| RICTOR | 125 (bp) | |
| F | 5′-GCCAAACAGCTCACGGTTGTAG-3′ | |
| R | 5′-CCAGATGAAGCATTGAGCCACTG-3′ | |
| GAPDH | 131 (bp) | |
| F | GTCTCCTCTGACTTCAACAGCG | |
| R | ACCACCCTGTTGCTGTAGCCAA |
mTOR, mammalian target of rapamycin; RPTOR, regulatory-associated protein of mTOR complex I; RICTOR, rapamycin-insensitive companion of mTOR; F, forward; R, reverse.