Figure 4.
MicroRNA-466 overexpression suppresses ROCK2 expression in HCC cell lines SNU-398 and SNU-182. (A) The relative expression levels of miRNA-466 and ROCK2 were determined by RT-qPCR and western blotting, respectively, in HCC cells following transfection with miRNA-466 mimic. (B) The relative expression levels of miRNA-466 and ROCK2 were determined by RT-qPCR and western blotting, respectively, in HCC cell lines following transfection with ROCK2 expression vector. *P<0.05 as indicated. HCC. ROCK2, rho associated coiled-coil containing protein kinase 2; HCC, hepatocellular carcinoma; RT-qPCR, reverse transcription-quantitative PCR.