Figure 3.

(A) Immunochemical stains for DAPI (blue), von Willebrand factor (red) and vimentin (green) for isolated CFs from 1–3 day old neonatal SD rats (fluorescence microscope, ×200; scale bar, 50 µm). CFs were determined by positive staining for vimentin and negative staining for von Willebrand factor. The purity of the CFs was >95%. (B) TMZ reduced the protein expression of collagen-I and collagen-III in cardiac fibroblasts. (C) TMZ reduced the content of hydroxyproline in the cell supernatant. Values are expressed as the mean ± standard deviation (n=3). *P<0.05, **P<0.01 and ***P<0.001 vs. NG; ^#P<0.05, ^##P<0.01 and ^###P<0.001 vs. HG. NG, normal glucose; HG, high glucose; TMZ, trimetazidine.