Transcriptional changes associated with the knockdown (KD) corroborate a role for NT5C2 on protein translation. Volcano plots indicate nominally significant transcriptomic changes elicited by (A) small interfering RNA (siRNA) A and (B) siRNA B. (C) Venn diagrams indicating the number of genes differentially regulated by siRNA A and siRNA B and the overlapping gene set, which is unlikely to occur by chance, according to Fisher’s exact test (p < .001). (D) Gene ontology (GO) terms enriched within genes concordantly, differentially expressed in both KD conditions. The line indicates the significance threshold (−log10 q < .05). mRNA, messenger RNA; p-val, p value.