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. 2019 Jul 1;50(1):11–24.e10. doi: 10.1016/j.devcel.2019.04.036

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© 2019 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Spd2 Phosphomutants Influence Spd2 and Fzr Centriolar Recruitment without Impacting on Cnn or Plp

(A) Immunostaining of Spd2^WT, Spd2^DE, and Spd2^AA early interphase NBs for Cnn (red) and Plp (green). DNA in blue. Insets show higher magnifications of each centriole. Scale, 4 μm. See also Figures S1 and S6.

(B and C) Dot plot showing Cnn and Plp fluorescent intensity levels on centrioles in the indicated genotypes (Cnn− Spd2^WT: Ctrl apical 0.7 ± 0.06, Ctrl basal 0.3 ± 0.04; Spd2^DE: Cent1 0.7 ± 0.1, Cent2 0.4 ± 0.08; Spd2^AA: Cent1 1.4 ± 0.4, Cent2 0.6 ± 0.2 and Plp − Spd2^WT: Ctrl apical − 0.01 ± 0.06, Ctrl basal 0.2 ± 0.07; Spd2^DE: Cent1 0.06 ± 0.05, Cent2 0.3 ± 0.09; Spd2^AA: Cent1 0.3 ± 0.07, Cent2 0.5 ± 0.1). Error bars represent means ± SD from at least 3 independent experiments. SS was assessed by unpaired t test.

(D) Images of Spd2^WT, Spd2^DE, and Spd2^AA early interphase NBs showing Spd2 (red) and Fzr (green). DNA, blue. Insets show higher magnifications of each centriole. Scale, 4 μm.

(E and F) Dot plot showing Spd2 and Fzr fluorescent intensity levels on centrioles in the indicated genotypes. (Spd2 − Spd2^WT: Ctrl apical 1.4 ± 0.2, Ctrl basal 0.6 ± 0.1; Spd2^DE: Cent1 0.6 ± 0.1, Cent2 0.3 ± 0.06; Spd2^AA: Cent1 2.3 ± 0.6, Cent2 2.1 ± 0.5 and Fzr- Spd2^WT: Ctrl apical 0.8 ± 0.07, Ctrl basal 0.4 ± 0.06; Spd2^DE: Cent1 0.1 ± 0.02, Cent2 0.06 ± 0.04; Spd2^AA: Cent1 0.7 ± 0.1, Cent2 0.4 ± 0.07). Error bars represent means ± SD from at least 3 independent experiments. SS was assessed by unpaired t test.