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. 2019 Jun 26;18(2):1291–1298. doi: 10.3892/etm.2019.7713

Figure 3.

Figure 3.

miR-873 directly targets HDGF in EC cells. (A) The miR-873 wild-type binding site in the 3′-UTR of HDGF wt and its mut form. The mut sequence is underlined. (B) Luciferase reporter assay analysis in HEC-59 and HEC-1B cells following the co-transfection of pmirGLO-HDGF-3′-UTR wt and miR-873 mimics. *P<0.05 vs. miR-NC. (C) RT-qPCR analysis of HDGF mRNA expression in 47 pairs of EC tissues and adjacent normal endometrial tissues. *P<0.05 vs. normal endometrial tissues. (D) Spearman's correlation analysis of miR-873 and HDGF mRNA in EC tissues. (E) RT-qPCR analysis of HDGF mRNA and (F) western blot analysis of protein expression in HEC-59 and HEC-1B cells after transfection with miR-873 mimics or a miR-NC. *P<0.05 vs. miR-NC. miR, microRNA; HDGF, hepatoma-derived growth factor; EC, endometrial cancer; UTR, untranslated region; NC, negative control; RT-q, reverse transcription-quantitative; wt, wild-type; mut, mutant.