Figure 6.

Effect of melatonin and PB on zebrafish sleep-like behaviour. Effect of melatonin (10 mg/kg; positive control) and PB (10 and 30 mg/Kg) on early life DEX treated (2 and 20 mg/ml) adult zebrafish on sleep-like behaviour, which is characterized by (A) total distance travelled, (B) % time spent in the upper zone of the tank (place preference) and (C) inactive duration (quiescent phase). The data were analyzed by one way ANOVA followed by dunnett post-hoc test, and was represented as the mean ± SEM, n=8. *P<0.05; **P<0.01; ***P<0.001 vs. the DEX-2 Vehicle Group. ^#P<0.05; ^##P<0.01; ^###P<0.001 vs. the DEX-20 vehicle group. PB, Piper betle L. leaf extract; DEX, dexamthasone; ANOVA, analaysis of variance; SEM, standard error of the mean.