Skip to main content
. 2019 Apr 30;47(12):6130–6144. doi: 10.1093/nar/gkz312

Figure 1.

Figure 1.

Zfp217 depletion impairs adipogenesis of 3T3L1 cells. (A) The mRNA and protein expression levels of Zfp217 were detected in 3T3L1 cells treated with siRNA for 36 h (n = 3). (B) The adipogenic phenotypes of 3T3L1 cells transfected with siZfp217 or siCtrl after MDI induction for 6 days were assessed by ORO staining; magnification: 200×. 3T3L1 cells were differentiated into adipocytes using MDI cocktail medium. (C) mRNA levels of adipogenic key genes PPARγ, aP2, LPL and Adiponectin at day 6 were detected by QPCR (n = 3). (D) Protein levels of PPARγ and aP2 at day 6 were detected by western blot (n = 3). (E) Schematic representation of knockout of Zfp217. (F) The adipogenic phenotypes of WT and Zfp217−/- cells with the treatment of MDI for 6 days. (G) mRNA levels of PPARγ, aP2, LPL and Adiponectin from WT and Zfp217−/- cells with the treatment of MDI for 6 days (n = 3). (H) Protein levels of PPARγ and aP2 from WT and Zfp217−/- cells with the treatment of MDI for 6 days (n = 3). (I) Protein expression of Zfp217 in MEF-Zfp217+/- cells. (J) The adipogenic phenotypes of MEF-Zfp217+/- cells with the treatment of MDI for 6 days. (K) mRNA expression of adipogenic key genes in MEF-Zfp217+/- cells with the treatment of MDI for 6 days (n = 3). (l) protein expression of adipogenic key genes in MEF-Zfp217+/- cells with the treatment of MDI for 6 days (n = 3). Presented as means ± SD ( **P < 0.01).