Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Apr 30;47(12):6130–6144. doi: 10.1093/nar/gkz312

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

PMC Copyright notice

Figure 2. — Zfp217 depletion increases m⁶A modification in 3T3L1 cells. (A) Dot blot was used to detect the m⁶A modification after knockdown of Zfp217. Methylene blue staining was used as a loading control (n = 3). (B) m⁶A/A ratio in polyadenylated RNA was measured from control and Zfp217 knockdown 3T3L1 cells using LC-MS/MS (n = 2). (C) m⁶A immunostaining of 3T3L1 cells transfected with siCtrl and siZfp217. Nucleus was stained with DAPI (magnification: 200×). (D) Dot blot was used to detect the m⁶A modification after knockout of Zfp217 (n = 3). (E) m⁶A modification levels were measured after knockout of Zfp217 using LC-MS/MS (n = 2). (F) m⁶A level during adipogenesis of 3T3L1 (n = 3). (G) Dot blot was used to detect the m⁶A modification in MEF-Zfp217^+/- cells (n = 3). (H) m⁶A modification level was measured in MEF-Zfp217^+/− cells using LC-MS/MS (n = 2). Presented as means ± SD (**P < 0.01).