Figure 5.
Effect of Zfp217 deletion on the expression of target genes. (A) Validation of Ccdc141, Hspa1a and Efcab11 mRNA levels from RNA-seq data by QPCR (n = 3). (B) Validation of m6A modification in Ccdc141, Hspa1a and Efcab11 mRNA by MeRIP-QPCR (n = 3). (C) The adipogenic phenotypes of 3T3L1 cells transfected with siCcdc141, siHspa1a, siEfcab11 or siCtrl after MDI induction for 6 days were assessed by ORO staining. (D) mRNA expression levels of adipogenic key genes PPARγ, aP2, LPL and Adiponectin were detected by QPCR (n = 3). (E) Protein levels of PPARγ and aP2 were detected by western blot (n = 3).Presented as means ± SD (*P < 0.05, **P < 0.01).