Figure 7.

Zfp217 influences competition between YTHDF2 and FTO in m⁶A-binding sites. (A) Dot blot was used to measure the effect of overexpression of FTO, Zfp217 or YTHDF2 in 3T3L1 cells on m⁶A modification (n = 3). (B) Schematic representation of RNA pull down. (C) Synthesized mRNA with m⁶A was incubated with YTHDF2 in the absence or presence of Zfp217, followed by RNA pull-down and western blot (n = 3). (D) Synthesized mRNA with m⁶A was incubated with FTO in the absence or presence of YTHDF2 and Zfp217, followed by RNA pull-down and western blot (n = 3). (E) mRNA of 3T3L1 cells was incubated with FTO, Zfp217 and YTHDF2, followed by m⁶A dot blot (n = 3). (F) Zfp217 directly interacts with YTHDF2 in a dose-dependent manner by LSPR. (G) Zfp217 blocked the binding capacity between YTHDF2 and m⁶A ssRNA by LSPR.