Table 2.
Melting temperatures (T1/2) measured by FRET-melting in K+ 1 buffer (Li cacodylate 10 mM, KCl 1mM, LiCl 99 mM)
| Sequence | T1/2 (°C) | T1/2 (°C) 1eq PhenDC3 | ΔT1/2 (°C) 1eq PhenDC3 | T1/2 (°C) 2eq PhenDC3 | ΔT1/2 (°C) 2eq PhenDC3 | T1/2 (°C) 5eq PhenDC3 | ΔT1/2 (°C) 5eq PhenDC3 |
|---|---|---|---|---|---|---|---|
| F-(GGGA)4-T | 47.9 ± 2.4** | 64.8 ± 1.0 | 17.9 | 72.1 ± 0.6 | 25.2 | >83.0 ± 0.4 | >36.1 |
| F-(GGGC)4-T | 64.5 ± 0.5 | 76.7 ± 1.0 | 12.2 | 82.6 ± 1.4 | 18.1 | >88.6 ± 0.3 | >24.1 |
| F-(GGGG)4-T | 63.9 ± 0.6* | 72.7 ± 1.2 | 8.8 | 78.3 ± 0.6 | 14.4 | >85.9 ± 1.0 | >22.0 |
| F-(GGGT)4-T | 63.7 ± 0.5 | 80.1 ± 0.3 | 16.4 | 84.5 ± 1.1 | 20.8 | >89.6 ± 0.2 | >25.9 |
*indicated approximate value as the curves show no clear transition, indicating the presence of several species (intra- and intermolecular G-quadruplexes).
**T1/2 values with high standard deviation (likely due to the existence of several species in equilibrium as suggested by the drift of the melting curve at low temperatures).