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. 2019 Mar 28;47(12):e68. doi: 10.1093/nar/gkz212

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© The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Alignment of pure experimental sample of the bacteriophage lambda genome. DNA was labelled with Atto647N using M.TaqI to direct labelling, combed and imaged. 38,000 candidate DNA barcodes were extracted from the images. (A) After filtering the experimental data, 1077 barcodes are identified for further analysis. (B and C) The fluorescence intensity profile of each experimental DNA barcode (red) is cross-correlated with a reference molecule (blue) to find the optimal stretch and alignment of the data. (D) Selected barcodes (368) with an alignment weight greater than the threshold. At the bottom of the image is shown the mean experimental barcode, the mean with the background removed and the reference barcode (top to bottom). (E) Alignment weight for all experimental barcodes is calculated and a threshold (of 0.7 in this case) is applied (black line). (F) Plot of mean experimental barcode (red) against the reference barcode (blue), with the number of barcodes (black).