Figure 4.

Specificity of TMM reporters 4–6 for mycomembrane-containing members of the Corynebacterineae suborder. Msmeg, Cglut, Bsub, or E. coli were incubated with 4 (25 μM), 5 (25 μM), 6 (100 μM), or left untreated for 4 h, then the cells were reacted with DBCO-488 (for 4), tetrazine-Cy3 (for 5), or left untreated (for 6), and analyzed by (A) flow cytometry and (B) fluorescence microscopy. In (A), error bars denote the standard deviation of three replicate experiments. In (B), scale bar (5 μm) at lower right applies to all images; grayscale images are transmitted light images.