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. 2019 Jun 13;212(3):757–771. doi: 10.1534/genetics.119.302237

Figure 3.

Figure 3

Effects of nicotinamide on heat-shock-mediated [PSI+] destabilization and mating. (A) Addition of nicotinamide (NAM) at the concentration of 10 mM does not decrease [PSI+] destabilization by mild heat shock in the wild-type (WT) strain. Colony numbers are shown in Table S8. (B) The sir2Δ strain is not capable of mating both in the absence of and in the presence of NAM, while the isogenic WT strain is capable of mating in the absence but not in the presence of NAM. Mating was detected in a combination with the tester WT strain of the opposite mating type (as indicated), by growth on synthetic medium selective for diploids (SD). Neither strain was capable of mating with the tester strain of the same mating type (data not shown). (C) Addition of NAM increases [PSI+] destabilization by mild heat shock in the sir2Δ strain. Colony numbers are shown in Table S9. Error bars in A and C indicate SD. Statistically significant differences in A and C are indicated by asterisks. Numbers of asterisks correspond to P values, as described in Figure 1 legend.