Zasp67 orthologs encode highly variable proteins. (A) Scatterplots of mRNA expression data from the Modencode project comparing the three Zasp genes to each other. The expression of Zasp52 and Zasp66 correlates (left panel). The expression of Zasp67 does not correlate with Zasp52 or Zasp66 (middle and right panels). Spearman rank correlation values are shown. RPKM, reads per kilobase of exon model per million mapped reads. (B) A plot of the relative expression (scaled RPKM) of all three Zasp genes against developmental time. E, embryonic stage; L, larval stage; PP, prepupal stage; P, pupal stage; A, adult; LBWM, larval body wall muscle; IFM, indirect flight muscle. Zasp67 expression is restricted to the pupal stages when IFM develop. (C) Table of all protein domains found associated with Zasp66 and Zasp67 orthologs. Accession numbers are provided in Table S3. (D) Histogram of Zasp66 and Zasp67 ortholog length frequencies. (E) Examples of Zasp66 and Zasp67 orthologs.