Skip to main content
. 2019 May 13;212(3):743–755. doi: 10.1534/genetics.119.302217

Figure 3.

Figure 3

Zasp67 expression is restricted to indirect flight muscles, where it localizes to Z-discs. (A) Confocal image of indirect flight muscles from Zasp67-GFP flies stained with phalloidin to visualize actin filaments (magenta in merged image). GFP fluorescence is shown in green (appearing white when overlapping with magenta in merged image). (B) Confocal image of indirect flight muscles from flies with Zasp67-GFP and Zasp52-mCherry alleles. Asterisks are drawn in (A and B) below selected Z-discs. (C) Profile plot of Zasp67 and Zasp52 fluorescence from a line extending over three Z-discs in (B). (D) Scatterplot of Zasp67 and Zasp52 fluorescence data from (B), with Pearson’s correlation value indicated. (E–J) Confocal images of jump muscles, leg muscles, and midgut muscles. Left panels show the merge of actin staining in magenta and GFP in green, right panels show only GFP. (E–G) Different adult muscles from Zasp67-GFP flies. (H–J) Different adult muscles from Zasp66-GFP flies. Bars are shown. IFM, indirect flight muscle.