Figure 6.

Zasp67 mutants have defective indirect flight muscle myofibrils. Confocal images from the indirect flight muscles of 3-day-old flies from different genotypes. Actin filaments are stained with phalloidin (magenta in merged image) and Zasp52-mCherry was used to mark the Z-discs (green in merged image). Asterisks are drawn below selected Z-discs. (A) Control flies have a stereotypic sarcomere organization. (B) Zasp67 null mutant indirect flight muscles showing broken and extended Z-discs. (C) Indirect flight muscles from a Zasp67 mutant transheterozygous over a deficiency uncovering the Zasp67 locus. (D) Zasp67 null mutant indirect flight muscles with a Zasp67-GFP rescue construct. (E) Zasp66 null mutant indirect flight muscles showing no obvious phenotypes. Bar, 5 µm.