Figure 7.

Zasp67 is required for myofibril maintenance rather than development. Confocal images of indirect flight muscles at different life stages. Actin filaments are stained with phalloidin (magenta in merged image) and Z-discs were labeled with anti-kettin (green in merged image). (A) Indirect flight muscles 96 hr after puparium formation (APF). Zasp67 null mutant muscles exhibit no phenotype compared to the wild-type control. (B) Indirect flight muscles of 2-day-old flies. At this stage, Zasp67 null mutant muscles present abnormal Z-discs for the first time. (C) Indirect flight muscles of 7-day-old flies. At this stage, a greater number of frayed myofibrils is present in Zasp67 null mutant flies. Bar, 5 µm.