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Sex effect on circulating Treg number in ESRD and healthy subjects. PBMCs from healthy subjects (n = 10) and ESRD patients with SLE (n = 12) and health problems (n = 14) were stained with anti-CD4, anti-CD25, and anti-foxP3 antibodies. The percentage of Tregs in participants was determined by flow cytometry (a-c) and later analyzed (d-f). The results of Mann-Whitney test revealed that the frequency of Tregs was lower in women than men. Each bar in (d-f) shows mean ± SEM. (*P < 0.05).
