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. Author manuscript; available in PMC: 2020 Jun 17.
Published in final edited form as: Curr Biol. 2019 May 30;29(12):2058–2065.e2. doi: 10.1016/j.cub.2019.05.010

Figure 3. Hyperpolarizing T4/T5 neurons eliminates object responses, depolarizing them induces visual valence reversal.

Figure 3

(A) Distribution of presynaptic and dendritic neurites of Tdc2-Gal4 neurons. Red = DenMark labeling; Green = synaptotagmin labeling; Blue = anti-BRP labeling. Me: medulla; Lo: lobula; LoP: lobula plate; SEZ: subesophgeal zone.

(B,B’) Genetic controls, enhancerlessless split-gal4 driving UAS-Chrimson, n=19. Mean ΔWBA (solid line) and SEM (shaded region) to an object (B) or bar (B’) in LED Off (black) or On (red). Inset: as in Figure 1.

(C,C’) Same as B for optogenetic depolarization of T4/T5 neurons, n=18 *** p<<0.01, Student’s paired t-test of the last 2 seconds.

(D,D’) Genetic controls, enhancerless split-Gal4 driving UAS-Kir2.1, for hyperpolarizing T4/T5 neurons. Mean ΔWBA (solid line) and SEM (shaded region) to an object (D) or bar (D’) in clean air (black) or ACV (red) n=13 *** p<<0.01. Inset: as in Figure 1.

(E,E’) Same as D, results of hyperpolarizing T4/T5 using Kir2.1, n=27 ** p<0.01, Student’s paired t-test of the last 2 seconds.

See also Figure S3.