| Component | Amount (μL) | Final concentration |
| DNA (step 9) | 50-100 | |
| NEB Buffer 2 | 15 | 1X |
| BSA | 1.5 | 1X |
| Spermidine | 1.5 | 0.5X |
| Restriction Enzymes (NEB; Reagents) | 30U each | |
| Water | Up to 150 μL |
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