| Component | Amount (μL) | Final concentration |
| DNA | 40 | |
| 5X second strand Buffer | 20 | 1X |
| 10 mM dNTP | 5 | 0.5 mM |
| 16 mM NAD | 1 | 160 μM |
| Water | 32.2 | |
| Total | 98.2 |
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