Component | Amount (μL) | Final concentration |
Sonicated DNA from step 43 | 40.5 | - |
NEB 10X end repair module Buffer | 5 | 1X |
ATP 10 mM | 2 | 0.4 mM |
NEBNext End repair module enzyme | 2.5 | - |
Total | 50 |
Component | Amount (μL) | Final concentration |
Sonicated DNA from step 43 | 40.5 | - |
NEB 10X end repair module Buffer | 5 | 1X |
ATP 10 mM | 2 | 0.4 mM |
NEBNext End repair module enzyme | 2.5 | - |
Total | 50 |