| Component | Amount (μL) | Final concentration |
| Sonicated DNA from step 43 | 40.5 | - |
| NEB 10X end repair module Buffer | 5 | 1X |
| ATP 10 mM | 2 | 0.4 mM |
| NEBNext End repair module enzyme | 2.5 | - |
| Total | 50 |
| Component | Amount (μL) | Final concentration |
| Sonicated DNA from step 43 | 40.5 | - |
| NEB 10X end repair module Buffer | 5 | 1X |
| ATP 10 mM | 2 | 0.4 mM |
| NEBNext End repair module enzyme | 2.5 | - |
| Total | 50 |