| Component | Amount (μL) | Final concentration |
| DNA from step 50 | 10 | - |
| PCR primer 1.0 P5 | 1 | 0.3 μM |
| PCR primer 2.0 P7 | 1 | 0.3 μM |
| Phusion master mix | 15 | 1X |
| Water | 3 | - |
| Total | 30 |
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