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a Percentage MFI deviation from the observed full bead dataset (≈ 60) as a function of bead number subsamples (10, 20, 30, 40 and 50) for gene 1, 2, 5 and 6. The vertical dotted line indicates the piece-wise regression cutoff point. b Distribution of the bead number thresholds, above which the percentage MFI, relative to the entire bead population MFI is no longer affected, defined by piece-wise linear regression. The 95th % bead number quantile is indicated with a vertical dashed line
