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. 2019 Jul 8;19:167. doi: 10.1186/s12906-019-2581-x

Table 1.

The primer pairs used in the qPCR assay and amplicon sizes

Gene Primer Sequences Product size (bp)
GAPDH

Forward 5′- AGCCTCAAGATCATCAGCAATGCC-3′

Reverse 5′- TGGACTGTGGTCATGAGTCCTTCC-3’

110
S100β

Forward 5’- GGAAGGGGTGAGACAAGGA-3′

Reverse 5′- GGTGGAAAACGTCGATGAG-3’

73
p75 NGFR

Forward 5’- AACAAGACCTCATAGCCAGCACGG −3′

Reverse 5′- AGCTGTTCCACCTCTTGAAGGC-3’

174
MBP

Forward 5’-CTTCAAGAACATTGTGACGCCTCG-3′

Reverse 5′- GTGAGCCGATTTATAGTCGGACG-3’

148
GFAP

Forward 5’-ACCTGCAGATTCGAGAAACCAGC-3′

Reverse 5′-ACATCCTTGTGCTCCTGCTTGG-3’

136

GAPDH Glyceraldehyde 3-phosphate dehydrogenase, p75 NGFR Low-affinity nerve growth factor receptor, MBP Myelin binding protein, GFAP Glial fibrillary acidic protein, bp Base pair