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. 2019 Jul 2;10:1479. doi: 10.3389/fimmu.2019.01479

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Copyright © 2019 Kiernan, Wang, Vanderplow, Cherukuri, Cahill and Watters.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Flow cytometry analysis of CD4⁺ cells in whole brains isolated from neonatal rats. (A) CD4⁺ cells were gated and analyzed for concomitant CD45 and CD11b expression in Nx and IH conditions. T cells exhibit high CD45 with low CD11b expression. (B) Approximately 3–4% of total live cells were CD4⁺, the majority of which were high in both CD11b and CD45 expression; microglia are CD11b⁺/CD45^low.