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. 2019 Jul 8;38:294. doi: 10.1186/s13046-019-1272-2

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — MAGEA3 inhibits autophagy in growth factor deprived pancreatic cancer cells. a-b Immunoblot showing decreased autophagic marker LC3-II in cells ectopically expressed with MAGEA3 (a) and the same LC3-II is increased upon MAGEA3 knockdown in BxPC3 cells (b). Star above the bands indicates the leaky expression of MAGEA3. c, d Immunofluorescence staining showing endogenous LC3-II puntae (c, scale bar = 25 μm) and the bar graph showing the corresponding quantification using ImageJ software (d); *** = p < 0.001, n = 10