Fig. 2.

Flubendazole inhibits STAT3 signaling pathway in a dose and time-dependent manner.(a) Cells were incubated with the flubendazole at different concentrations, as indicated, for 24 h, the cell lysates were prepared for western blot analysis to determine protein expression of P-STAT3, STAT3 and GAPDH, which was used as a loading control. The results are representative of three replicate experiments. (b) Flubendazole (1.2 μM) inhibits P-STAT3 in a time-dependent manner. (c) Cells were incubated with flubendazole for 24 h. Protein expression was determined by immunoblot analysis. GAPDH was used as a loading control. Results are representative of three independent experiments (**P < 0.01, ***P < 0.001, ****P < 0.0001)