Fig. 4.

Flubendazole induces cell apoptosis through activation of autophagy.(a) Three CRC cell lines were treated with DMSO or flubendazole (0.3, 0.6 and 1.2 μM), lysed and subjected to immunoblotting. (b) Cells were incubated with flubendazole for 24 h. Afterwards, western blot analysis of apoptosis-related proteins was performed. GAPDH was used as a loading control. Results are representative of three independent experiments