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. 2019 Jul 2;10:1511. doi: 10.3389/fimmu.2019.01511

Figure 5.

Figure 5

Melatonin reduced NLRP3, caspase-1 and ASC speck formation. N9 microglial cells were pretreated with melatonin (500 μM) for 1 h, then treated with LPS (1 μg) for 4 h and ATP (5 mM) for 1 h. (A,B) Pro-caspase-1 show no difference among groups. (A,C) Melatonin reduced cleaved caspase-1 in melatonin pretreated cells compared with that in LPS and ATP induced cells. (D) Melatonin pretreatment decreased caspase-1 activity compared to LPS and ATP induced cells. (E–G) Melatonin suppressed NLRP3 on both protein and mRNA level compared with in that LPS and ATP induced cells. (H) ASC speck formation was determined by confocal microscopy. (I) Melatonin significantly prevented ASC speck formation compared to LPS and ATP induced cells. Data are presented as mean ± S.E.M, n = 5. *p < 0.05, **p < 0.01 and ***p < 0.001 compared to control and #p < 0.05, ##p < 0.01 and ###p < 0.001 compared to LPS and ATP induced cells.