Fig. 2.

Inhibition of E. coli K88 adhesion by specific IgY. E. coli K88 was pretreated with specific IgY and the number of bacteria adhered to crude porcine jejunal (a) and ileal (b) mucus was determined by bacterial colony counts. FITC-labelled E. coli was incubated with 50 mg/mL of yolk powder or specific IgY. Bacterial adherence to jejunal (c) and ileal (d) mucus was measured by fluorescence intensity changes. Results were expressed as means ± SEM, n = 3, (*, P < 0.05; ***, P < 0.001). FITC-labelled E. coli was pre-treated with PBS (e), 50 mg/mL of yolk powder (f) or specific IgY (g). Immunofluorescence microscopy data show the adhesion of E. coli K88 (in Green) to jejunal mucus (magnification × 10). Data shown are generated from three independent experiments. The raw data was shown in Additional file 2: Figure S2