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Biochemical estimation of leukotriene B4 (LTB4) by competitive ELISA in control group of animals before (basal) and after incision (between 2 hr and day 3). Maximum increase was observed at 8 hours compared to basal level. The increase persisted up to day 3. Values (n = 4) are represented as mean ± standard error of mean. *P < 0.05, **P < 0.01, ***P < 0.001.
