Selective microglia depletion in slices after extended PLX5622 treatment had no effect on mature oligodendrocytes, astrocytes, or myelin protein. A. Slices were treated with control (DMSO) or 2 αM PLX5622 for 8 days, stained and imaged for Iba1, NG2, PLP-eGFP, GFAP and myelin protein PLP. B. Quantification of the number of NG2+ and PLP-eGFP+ cells in slices. Cell count was normalized to the DMSO control (CTR) slice. Statistical analyses were performed by unpaired Student’s t test, ns: no significance, n=3. Scale bars: 25 μm.