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. Author manuscript; available in PMC: 2020 Aug 1.
Published in final edited form as: Exp Neurol. 2019 Apr 25;318:32–41. doi: 10.1016/j.expneurol.2019.04.011

Fig. 4.

Fig. 4

PLX5622 or PLX3397 chow eliminated microglia in the CNS of adult mice. Adult PLP-eGFP mice aged 8-10 weeks old were fed either control chow or chow containing 1200 mg/Kg PLX5622 or 275 mg/Kg PLX3397 for 7 or 21 days. A. Representative confocal images of Iba1 immunostaining in corpus callosum (CC), cortex (CTX) and cerebellum (CB) of control mice (CTR), PLX5622-treated, or PLX3397-treated animals. B. Quantification of the number of Iba1+ cells for all groups in corpus callosum (CC), cortex (CTR), cerebellum (CB), striatum (STR) and spinal cord (SC). For corpus callosum, the number of positive cells was presented per mm2 in the region of interest (ROI). For other regions, the number of positive cells in each field of view (FOV) is reported. Statistical analyses were performed by one-way ANOVA between PLX treated groups at 7 days or 21 days and control (CTR), and by unpaired Student’s t test for comparisons between PLX5622 and PLX3397 at 7days. *: p<0.05, **: p<0.01, ***: p<0.001, ****: p< 0.0001, n=4. Scale bars: 100 μm.