Table 3.

Results from the second experiment. Assesment of pollen grains number on stigma after clearing and staining with aniline blue and the furthermost pollen tube tip position in pistil in samples collected at a different time in relation to anthesis and on a different number of days after pollination. Pooled data of SM 1219–1 crossed with HMC-1 or self-pollinated. A total of 60 flowers (180 ovules) were analyzed^a.

Pollination of stigma in relation to anthesis (days)	Collection time after pollination (hours)	Pollen grains number on stigma after aniline blue stainingb	Position in pistil of the furthermost pollen tube tipc
One day before anthesis (-1)	24	1.25e	2.33cde
	48	2.67ab	3.67bc
	72	3.00a	5.25a
Anthesis day (0)	24	2.58abc	4.50ab
	48	2.50abc	5.25a
	72	2.75ab	4.83ab
One day after anthesis (1)	24	1.58de	2.00def
	48	1.55de	1.64ef
	72	2.75ab	1.08efg
Two days after anthesis (2)	24	1.58de	0.92fg
	48	2.50abc	0.08g
	72	2.00cd	2.08def
Three days after anthesis (3)	24	2.25bc	3.08cd
	48	2.25bc	1.33efg
	72	2.27bc	2.00def
-1	Averaged across all collection times in relation to pollination day	2.26b	3.75b
0		2.61a	4.86a
1		1.97b	1.57cd
2		2.03b	1.03d
3		2.26b	2.14c
Averaged across all pollination times in relation to anthesis day	24	1.85b	2.57ab
	48	2.3a	2.40b
	72	2.5a	3.07a

^aFor each section of the Table, values with the same letter within each column were not statistically different at P < 0.05, based on the LSD test.

^bScoring for pollen grain number was as follows: 1 – No pollen grains; 2 – from 1 to 5 grains; 3 – from 6 to 24 grains; and 4 – 25 or more pollen grains.

^cAverage position of the furthermost pollen tube tip (PTT) was assessed at different anatomical points of the pistil. Score was based on the pistil anatomy as follows: 1-PTT at the stigma surface or its shallow growth into the transmitting tissue; 2-PTT in the transmitting tissue of the style; 3-PTT at the bottom of the style; 4-PTT in the obturator; 5-PTT in the nucellar beak; and 6-PTT at the vicinity of the embryo sac.